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Hepatic phosphoenolpyruvate carboxykinase gene expression is not repressed by dietary carbohydrates in rainbow trout (Oncorhynchus mykiss) ArchiMer
Panserat, Stéphane; Plagnes Juan, E; Breque, Jeanine; Kaushik, Sadasivam.
Phosphoenolpyruvate carboxykinase (PEPCK) is a rate-limiting enzyme in hepatic gluconeogenesis and therefore plays a central role in glucose homeostasis. The aim of this study was to analyse the nutritional regulation of PEPCK gene expression in rainbow trout (Oncorhynchus mykiss), which are known to use dietary carbohydrates poorly. A full-length hepatic PEPCK cDNA (2637 base pairs with one open reading frame putatively encoding a 635-residue protein) was cloned and found to be highly homologous to mammalian PEPCKs. The presence of a putative peptide signal specific to a mitochondrial-type PEPCK in the deduced amino acid sequence suggests that this PEPCK gene codes for a mitochondrial form, In gluconeogenic tissues such as liver, kidney and intestine,...
Tipo: Text Palavras-chave: Gluconeogenesis; Nutrition; Metabolism; Glucose; Oncorhynchus mykiss; Rainbow trout; Phosphoenolpyruvate carboxykinase.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-414.pdf
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Implications of the use of glycerin on fed of finishing pigs: review Sci. Agrar.Paran. / SAP
Corassa, Anderson; Leite, Rafaeli Gonçalves; Silva, Daniela Rocha da; Santos, Tatiane Izabel Silva dos.
Brazilian pig production has been highlighted worldwide, currently the country has occupied the fourth place both in production and export of pork. The the Brazilian governmen has required the addition of 7% biodiesel in fossil diesel, this requirement also increased the amount of glycerin which is a byproduct from production of biodiesel by transesterification. Glycerin has interesting nutritional characteristics for animal feed, and reduce the cost of diets to have energy value similar to corn. For each 90 m3 of biodiesel produced by transesterification of vegetable oils are generated 10 m3 of glycerin. Glycerol is a nutritional component of dietary fat (triglycerides) in the diet can be absorbed as monoglycerides constituent after a partial hydrolysis...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coproducts; Metabolism; Glycerol; Performance..
Ano: 2017 URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/14372
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In modelling effects of global warming, invalid assumptions lead to unrealistic projections ArchiMer
Lefevre, Sjannie; Mckenzie, David J.; Nilsson, Goeran E..
Tipo: Text Palavras-chave: Climate change; Fish; Growth; Metabolism; Modelling; Oxygen uptake; Scaling; Warming.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00426/53762/54697.pdf
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Increasing levels of phytase in diets formulated with reduced available phosphorus content supplied to male and female broilers Ciência Rural
Naves,Luciana de Paula; Rodrigues,Paulo Borges; Teixeira,Levy do Vale; Bertechini,Antônio Gilberto; Alvarenga,Renata Ribeiro; Bernardino,Verônica Maria Pereira; Santos,Luziane Moreira dos; Natividade,Yuri Pereira Efrem.
The reduction of the available phosphorus (avP) content in the broiler diet must be followed by the supplementation of adequate phytase. One experiment was conducted with broilers from 15 to 28 days of age to determine the level of necessary phytase when the mashed diet is formulated with 0.213% of avP to enable the results of performance, tibia ash content, and calcium (Ca) utilization similar to those determined for birds fed with diet formulated to meet their nutritional requirements. Thus, 120 broilers were distributed in (4+1) x2 factorial arrangement corresponding to four deficient diets in avP (0.213%) supplemented with phytase (0; 750; 1,500 or 2,250FTU kg-1) plus one positive control diet without phytase (0.426% of avP), supplied to male and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aviculture; Bone ash; Enzyme; Metabolism; Performance; Phytate.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000801479
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Increasing the rate of drying reduces metabolic imbalance, lipid peroxidation and critical water content in radicles of garden pea (Pisum sativum L.) Biol. Res.
Ntuli,Tobias M; Pammenter,Norman W; Berjak,Patricia.
Orthodox seeds become desiccation-sensitive as they undergo germination. As a result, germinating seeds serve as a model to study desiccation sensitivity in plant tissues. The effects of the rate of drying on the viability, respiratory metabolism and free radical processes were thus studied during dehydration and wet storage of radicles of Pisum sativum. For both drying regimes desiccation could be described by exponential and inverse modified functions. Viability, as assessed by germination capacity and tetrazolium staining, remained at 100% during rapid (< 24 h) desiccation. However, it declined sharply at c. 0.26 g g¹ dm following slow (c. 5 days) drying. Increasing the rate of dehydration thus lowered the critical water content for survival. Rapid...
Tipo: Journal article Palavras-chave: Antioxidant; Desiccation; Drying rate; Free radical processes; Lipid peroxidation; Metabolism; Respiration; Pisum sativum; Seed storage.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200002
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Influence of CH4 and H2S availability on symbiont distribution, carbon assimilation and transfer in the dual symbiotic vent mussel Bathymodiolus azoricus ArchiMer
Riou, V.; Halary, S.; Duperron, S.; Bouillon, S.; Elskens, M.; Bettencourt, R.; Santos, R. S.; Dehairs, F.; Colaco, A..
High densities of mussels of the genus Bathymodiolus are present at hydrothermal vents of the Mid-Atlantic Ridge. It was previously proposed that the chemistry at vent sites would affect their sulphide- and methane-oxidizing endosymbionts' abundance. In this study, we confirmed the latter assumption using fluorescence in situ hybridization on Bathymodiolus azoricus specimens maintained in a controlled laboratory environment at atmospheric pressure with one, both or none of the chemical substrates. A high level of symbiosis plasticity was observed, methane-oxidizers occupying between 4 and 39% of total bacterial area and both symbionts developing according to the presence or absence of their substrates. Using (HCO3-)-C-13 in the presence of sulphide, or...
Tipo: Text Palavras-chave: Mid-atlantic ridge; Hydrocarbon-seep mussel; Deep-sea mussels; Hydrothermal-vent; Puteoserpentis bivalvia; Mytilidae; Methane; Endosymbionts; Bacteria; Metabolism.
Ano: 2008 URL: https://archimer.ifremer.fr/doc/00207/31865/30271.pdf
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Influence of water temperature and oxygenation on the aerobic metabolic scope of Atlantic cod (Gadus morhua) ArchiMer
Claireaux, Guy; Webber, D; Lagardere, Jean-paul; Kerr, S.
Environmental influences (temperature and oxygenation) on cod metabolism and their impact on the ecology of this species were investigated. Limiting oxygen concentration curves (O-2 level ranging between 15 and 100% air saturation) were established at 2, 5 and 10 degreesC. The standard metabolic rate (SMR), the maximum metabolic rate and the metabolic score were then modelled as functions of temperature and/or oxygen saturation. The mean SMR at 2, 5 and 10 degreesC were 19.8 +/- 4.9, 30.8 +/- 6.1 and 54.3 +/- 4.1 mg O-2 h(-1) kg(-1). respectively. Between 2 and 5 degreesC, the active metabolic rate of cod almost doubled from 65 to 120 mg O-2 h(-1) kg(-1), to reach 177 mg O-2 h(-1) kg(-1) at 10 degreesC. In terms of metabolic scope (MS), the temperature...
Tipo: Text Palavras-chave: Oxygen; Temperature; Physiological ecology; Metabolism; Atlantic cod.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-428.pdf
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Ingestion of a moderate dose of alcohol enhances physical exercise-induced changes in blood lactate concentration BJMBR
Teixeira-Coelho,F.; Santos,D.F.C.; Santos,G.A.; Sousa,T.F.; Moreira,S.R.; Souza,M.V.C.; Wanner,S.P..
The consumption of alcoholic beverages influences carbohydrate and lipid metabolism, although it is not yet clear whether metabolism during physical exercise at different intensities is also affected. This was the objective of the present study. Eight young and healthy volunteers performed a treadmill test to identify the running speed corresponding to a lactate concentration of 4 mM (S4mM). At least 48 h later, they were subjected to two experimental trials (non-alcohol or alcohol) in which they performed two 1-km running sessions at the following intensities: 1) S4mM; 2) 15% above S4mM. In both trials, blood lactate, triglycerides, and glucose concentrations were measured before and after exercise. The acute alcohol intake increased triglycerides, but...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endurance; Ethanol; Glucose; Lactate; Metabolism; Treadmill running.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000400601
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Insulin addition to swine semen diluted and cooled at 15 ºC R. Bras. Zootec.
Cunha,Evandro César Pereira; Zangeronimo,Márcio Gilberto; Murgas,Luis David Solis; Braga,Douglas Evangelista; Pereira,Bárbara Azevedo; Rocha,Luiz Gustavo Pessoa; Faria,Bruno Generoso; Pereira,Luciano José.
The objective of this study was to evaluate the effect of adding different doses of insulin to swine semen processed and stored at 15 ºC. The experiment used sixteen ejaculates from four commercial breeding pigs, distributed in a randomized block design (ejaculate) with split plot along time (0, 24, 48 and 72 hours of storage) with four treatments (insulin levels - 0.0 4.0 8.0 and 12.0 IU per dose) and 16 repetitions. The experimental unit was made of two insemination doses of 100 mL each, with 3×10(9) spermatozoids. Insulin used was NPH-human, added at the time of processing the doses. The addition of insulin did not affect motility, sperm viability, the percentage of abnormal cells, the osmotic resistance or the degradation rate of motility in 120...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Artificial insemination; Boar; Hormone; Metabolism; Sperm; Reproduction.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000400031
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Intake, nutrient apparent digestibility and ruminal constituents of sheep fed diets with canola, sunflower or castor oils R. Bras. Zootec.
Maia,Michelle de Oliveira; Susin,Ivanete; Ferreira,Evandro Maia; Nolli,Cristine Paduan; Gentil,Renato Shinkai; Pires,Alexandre Vaz; Mourão,Gerson Barreto.
The objective in this experiment was to determine the effects of feeding diets with canola, sunflower or castor oils on intake, nutrient apparent digestibility and ruminal constituents of crossbred Dorper × Santa Inês sheep. Four rumen-cannulated animals of 90.2±11.4 kg average body weight were assigned to a 4 × 4 latin square. Animals remained individually in cages for the metabolism assay and were fed diets containing roughage at 500 g/kg and concentrate based on ground corn and soybean meal also at 500 g/kg. No oil was added to the control diet, whereas the others had canola, sunflower or castor oils at 30 g/kg (DM basis). There was no difference for the intake of DM and nutrients, except for ether extract, which was greater when animals received oil....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ammonia; Lipids; Metabolism; Short-chain fatty acids.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001100008
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Intermittent sustained swimming in 'matrinxã' Brycon amazonicus (Bryconidae: Bryconinae): hematological and metabolic responses Neotropical Ichthyology
Fabrizzi,Fernando; Moraes,Gilberto; Hackbarth,Araceli; Almeida,Luciana Cristina de; Arbeláez-Rojas,Gustavo; Nunes,Cleujosí da Silva.
In fish, studies on a wide variety of physiological effects of exercise have been reported since a long time. It has been attributed special attention to some types of exercise, however, its application as a healthful practice in the rearing and welfare of farming fish is rising in last few years. In this particular, long-term intermittent sustained swimming (ISS) has been not yet explored. In this work, the freshwater fish Brycon amazonicus was submitted to (ISS) for 30 days at velocity of 1.0 body-length sec-1 for 12h interspaced by 12h under still water. Hematology and metabolism were evaluated. Exercised fish decreased 30% the erythrocyte number and hemoglobin was unvaried. The stores of liver glycogen and muscular triacylglycerol (TAG) were increased...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adaptation; Exercise; Fish; Glycogen; Metabolism; Triacylglycerol.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000200425
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Involvement of the TRPV1 channel in the modulation of spontaneous locomotor activity, physical performance and physical exercise-induced physiological responses BJMBR
Hudson,A.S.R.; Kunstetter,A.C.; Damasceno,W.C.; Wanner,S.P..
Physical exercise triggers coordinated physiological responses to meet the augmented metabolic demand of contracting muscles. To provide adequate responses, the brain must receive sensory information about the physiological status of peripheral tissues and organs, such as changes in osmolality, temperature and pH. Most of the receptors involved in these afferent pathways express ion channels, including transient receptor potential (TRP) channels, which are usually activated by more than one type of stimulus and are therefore considered polymodal receptors. Among these TRP channels, the TRPV1 channel (transient receptor potential vanilloid type 1 or capsaicin receptor) has well-documented functions in the modulation of pain sensation and thermoregulatory...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Afferent; Capsaicin; Hyperthermia; Metabolism; Physical activity; Thermoregulation.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000600301
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L'absorption intestinale des lipides chez la truite arc-en-ciel (Salmo gairdneri ) ArchiMer
Vernier, J; Sire, M.
Though the absorption of dietary lipids is much slower in S. gairdneri than in mammals, the mechanism does not differ fundamentally. The long chain fatty acids resulting from intraluminal hydrolysis are esterified by the epithelial cells and transferred as VLDL-like particles and chylomicrons to lymph. The size of granules depends on the nature of the ingested fatty acids. The intracellular stages of this lipoprotein synthesis are defined.
Tipo: Text Palavras-chave: Salmonidae; Pisces; Lymph; Fatty acids; Nutrition; Lipoproteins; Lipids; Intestines; Food absorption; Metabolism.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1245.pdf
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L'adaptation ecophysiologique et sanitaire de l'ecrevisse turque Astacus leptodactylus a des milieux saumatres: Contribution a l'astaciculture ArchiMer
Barbe, D; Lautier, J; Vernet, G; Vey, A.
This influence of salt media on different aspects of biological cycle and on most common pathogenic agents of Turkish freshwater crayfish Astacus leptodactylus is investigated survival and growing of juveniles O super(+) and intermediary individuals 1 super(+) are also studied. Total osmotic metabolism and reproduction, have been recorded. It appears that youngest individuals are particularly sensitive to low salinities (7 ppt.). Intermediary ones are more resistant nevertheless salinity does not improve neither survival nor growing. Copulation, laying and pleopod egg fixation are particularly affected by salt waters. The only promising application of salinity parameter on astaciculture could be whether prophylactic or curative treatment with concentrated...
Tipo: Text Palavras-chave: Astacus leptodactylus; Malacostraca; Infectious diseases; Sexual reproduction; Growth; Mortality; Survival; Osmotic adaptations; Crayfish culture; Pathology; Metabolism; Salinity; Ecophysiology; Astaciculture; Régulation osmotique; Reproduction; Survie; Croissance; Pathologie; Ecophysiologie; Salinité; Astacus leptodactylus; Ecrevisse.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1185.pdf
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Levels of true digestible lisiane and metabolizable energy for growing pigs: effect on nitrogen and energy balance Ciência e Agrotecnologia
Chiaradia,Rafael Coutinho Finamor; Zangeronimo,Márcio Gilberto; Fialho,Elias Tadeu; Lima,José Augusto de Freitas; Rodrigues,Paulo Borges; Sousa,Raimundo Vicente de.
An experiment was conducted at the Department of Animal Husbandry of Universidade Federal de Lavras aiming to study the nitrogen and energy balance in diets with reduced crude protein rates (CP) and different levels of energy and lysine for growing pigs. 52 castrated pigs (initial weight = 36.13 ± 2.8 kg) of high genetic potential were used, housed individually in metabolism cages (experimental unit). The treatments consisted of 12 diets with three levels of metabolizable energy (ME) (3060, 3230 and 3400 kcal / kg) and four of true digestible lysine (TDL) (0.7, 0.9, 1.1 and 1, 3%) with 14% CP plus a control diet with 3230 kcal ME / kg, 1.03% TDL and 18% CP. We used a DBC in time 3x4 +1 factorial design with four replications to verify the absorbed nitrogen...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Synthetic amino acids; Nutrition; Metabolism; Ideal protein.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542009000600028
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Live weight and metabolic hormone profile in steers moved in drives R. Bras. Zootec.
D'Oliveira,Marcella Cândia; Souza,Maria Inês Lenz; Ítavo,Camila Celeste Brandão Ferreira; Corrêa Filho,Ruy Alberto Caetano; Morais,Maria da Graça; Negrão,João Alberto; Franco,Gumercindo Loriano.
The objective was to determine the effects of driving on the growth of steers during 55 days of moving by drive and 84 days after the arrival. Twenty-five steers were randomly chosen from a herd of 1,000 Nellore cattle, which were evaluated in two periods: the driving period, in which animals were moved a distance of 700 km on foot for 55 d; and the post-driving period, which lasted 84 d. Serum samples were obtained for hormone dosing (T3, T4, cortisol, and IGF-I) by radioimmunoassay using commercial kits. The data was collected at different time points during the experiment. No changes in live weight were observed between days 0 and 55. During the post-driving period, on the 28th, 56th, and 84th d, the average weights were 226.10 kg, 224.28 kg, and 242.6...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Compensatory gain; Metabolism; Post-driving; Transportation.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014001000551
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Low pH reduced survival of the oyster Crassostrea gigas exposed to the Ostreid herpesvirus 1 by altering the metabolic response of the host ArchiMer
Fuhrmann, Marine; Richard, Gaëlle; Quere, Claudie; Petton, Bruno; Pernet, Fabrice.
Environmental change in the marine realm has been accompanied by emerging diseases as new pathogens evolve to take advantage of hosts weakened by environmental stress. Here we investigated how an exposure to reduced seawater pH influenced the response of the oyster Crassostrea gigas to an infection by the Ostreid herpesvirus type I (OsHV-1). Oysters were acclimated at pH 8.1 or pH 7.8 and then exposed to OsHV-1. Their survival was monitored and oyster tissues were sampled for biochemical analyses. The survival of oysters exposed to OsHV-1 at pH 7.8 was lower (33.5%) than that of their counterparts at pH 8.1 (44.8%) whereas levels of OsHV-1 DNA were similar. Energetic reserves, fatty acid composition and prostaglandin levels in oyster did not vary...
Tipo: Text Palavras-chave: Acidification; Bivalve; Disease; Environment; Metabolism; Mortality risk.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00472/58334/60887.pdf
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Lysine catabolism: flow, metabolic role and regulation Braz. J. Plant Physiol.
Fornazier,Ricardo Francisco; Azevedo,Ricardo Antunes; Ferreira,Renato Rodrigues; Varisi,Vanderlei Aparecido.
Lysine is an essential amino acid, synthesized in plants in the aspartic acid pathway. The lysine catabolism is performed by the action of two consecutive enzymes, lysine 2-oxoglutarate reductase (LOR) and saccharopine dehydrogenase (SDH). The steady state of lysine is controlled by both, synthesis and catabolism rates, with the final soluble lysine concentration in cereal seeds a direct result of these processes. In the last 40 years, the enzymes involved in lysine biosynthesis have been purified and characterized from some plant species such as carrot, maize, barley, rice, and coix. Recent reports have revealed that lysine degradation might be related to various physiological processes, for instance growth, development and response to environmental...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acids; Lysine; Metabolism; Mutants.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202003000100002
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Maintenance of discrete bacterial populations in adjacent marine habitats. ArchiMer
Robb, F; Muir, D; Davis, C.
Some mechanisms of selection which operate on marine Vibrio and Pseudomonas strains during survival in dilute environments have been examined. Mannitol uptake and utilisation systems are more efficient in the Vibrio strain compared with the Pseudomonas strain during starvation survival. A Vibrio mutant strain lacking mannitol uptake showed no greater loss of viability during starvation survival than mannitol positive strains. The Vibrio strain appears to be constitutive with respect to mannitol uptake systems, whereas the Pseudomonas strain examined is inducible. Active uptake systems for glucose, glutamate, alanine and aspartic acid were efficiently maintained during starvation survival in the Vibrio strain. Results suggest that membrane uptake systems...
Tipo: Text Palavras-chave: Pseudomonas; Vibrio; Bacteria; Physiology; Alcohols; Food conversion; Survival; Starvation; Metabolism.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-960.pdf
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Mastitis in the transition period: identification of potential blood markers Arq. Bras. Med. Vet. Zootec.
Santos,K.R.; Souza,F.N.; Blagitz,M.G.; Batista,C.F.; Bertagnon,H.G.; Gomes,R.C.; Molinari,P.C.C.; Diniz,S.A.; Silva,M.X.; Haddad,J.P.A.; Heinemann,M.B.; Libera,A.M.M.P.D..
ABSTRACT This study identified potential blood markers associated with mastitis in dairy cows under different dry therapies during the transition period, using a logistic regression model. Thirty-four Holstein dairy cows were divided into three groups: untreated controls (13 cows, 42 quarters); animals that received an antimicrobial at drying-off (10 cows, 40 quarters); and animals that were administered an internal teat sealant at drying-off (11 cow, 44 quarters). Blood and quarter milk samples were collected 60 days before the expected day of calving, on the day of calving, and three, seven, 15, 21, and 30 days after calving. Milk samples were submitted for bacteriological analysis and somatic cell count. Blood samples were collected for analyses of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Intramammary infection; Risk factor; Drying-off; Periparturient period; Metabolism.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000401120
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